image

Liquid Chromatograpy – mass spectroscopy adalah dua  alat yang digabungkan menjadi satu, dimana berfungsi untuk memisahkan beberapa senyawa atau campuran senyawa berdasarkan kepolarannya (prinsip kerja kromatograpi), dimana setelah campuran senyawa tersebut terpisah, maka senyawa yang murni tersebut akan diidentifikasi berat moleculnya. Berbeda dengan Gas Chromatograpy – mass spectroscopy, LC-MS pada output datanya sangat jarang sekali terjadi pola fragmentasi, dikarenakan tidak ada proses fragmentasi. Sehingga yang didapatkan adalah berat molekul ditambah beberapa muatan ditambah lagi berat molekul pelarut (terkadang).

ESI adalah salah satu methode untuk mendapatkan berat molekul yang digunakan dalam LC-MS dimana jika pada metode biasanya (lupa-red) menggunakan fragmentasi (pemecahan molekul), maka pada metode ESI menggunakan spray (Penyemprotan). AKibatnya tidak akan ditemukan fragmen –fragmen dari molekul tersebut.

Adapun cara kerja liquid chromatograpi adalah sama dengan HPLC atau liquid chromatograpy lain pada umumnya. Sedangkan kerja Mass spectroscopy metode ESI  adalah sebagai berikut : 

image 

Gambar 1. Proses pemisahan analite pada Liquid Chromatograpy sampai dengan sprayer (penyemprotan oleh spray needle tip)

1. Analyte bersama dengan eluent dari syringe pump atau LC masuk ke dalam cappilary.

Di dalam kappilary terdapat anoda (kutup negatif) pada taylor cone dan katoda (kutup negatif) didekat masukkan analyte dan eluent. Kutup ini berfungsi agar muatan yang berkumpul pada taylor cone adalah muatan positif sehingga nantinya saat terjadi penyemprotan dan terbentuk droplet (tetes – tetes) tidak bergabung – gabung menjadi droplet yang lebih besar lagi.

2.  Analylet dan solven(eluent) di semprotkan (spray) melalui taylor cone.

Akan terbentuk droplet – droplet dimana droplet – droplet itu akan mengalami tahap evaporasi solven untuk mengurangi solven yang menempel di analyte. Karena suatu saat , apabila terjadi evaporasi secara terus menerus maka solven yang meliputi analyte terkungkung dalam muatan positif yang berlebih, dalam bahasa inggris tahap seperti ini disebut the ‘rayleigh’  limit is reached, maka akan terjadi explosion yang disebut coulombic explosion dimana akan terjadi pemecahan droplet (tetesan) tadi. Ada beberapa kemungkinan yang terjadi pada droplet – droplet tersebut .

a. Yang pertama analyte akan tertambahi satu muatan positif

b. yang kedua analyte akan tertambahi beberapa muatan positif

c. yang ketiga analyte akan tertambahi satu muatan positif dan satu molekul solven

d. yang keempat analyte akan tertambahi satu muatan positif dan beberapa molekul solven

e. yang kelima analyte akant tertambahi beberapa muatan positif dan beberapa molekul solven.

image

Gambar 3.Proses Setelah analite dipisahkan oleh Liquid Cromatography

3. Droplet yang mengalami coulombic exsploison tersebut akan masuk ke dalam cone dimana di sisi kiri dan kanannya sudah mengalir gas Nitrogen (N2). Gas ini berfungsi agar analyte yang terjadi tadi stabil dalam bentuknya dan tidak terganggu oleh pengaruh gas oksigen. Droplet masuk ke dalam cappilary transfer lalu akan di analisis melalui mass spectrometer.

image

Gambar 3. Proses Droplet berubah menjadi analyte yang siap untuk di mass spectroscopy

image

gambar 4. Penjelasan datangnya muatan positif pada droplet – droplet LC-MS

image

Gambar 5. Penjelasan kenapa pada LC-MS solven harus di buat muatan positif

 

image gambar 6. Penjelasan kenapa bukan ion negatif yang digunakan

image gambar 7. Penjelasan ion molekul semu apa saja yang terjadi /yang bisa muncul pada output data LC-MS

image

gambar8. Contoh spektra dan hasil

image

image

Gambar 9. Kerugian yang dihasilkan dibandingkan dengan LC-MS

image

Gambar 10. Kesimpulan , diambil dari presented Jessica & Gilement, 2002.

DAFTAR PUSTAKA

  • Adamovics, & John.A, 1997, Chromatographic Analysis of Pharmaceutical, Marcel dekker Inc, New York.

  • Ardrey, R. E., 2003, Liquid chromatography-mass spectrometry: an introduction, 185, John Wiley & Sons, New York.

  • De Hoffmann, E., & Stroobant, V.,2007, Mass spectrometry: principles and applications, 226-227, Wiley-Interscience, New York.

  • Jesicca & Gilment, 2002, Electrospray ionization Mass Spectrometry, Presented at 17 september 2002.

10,729 Comments on LIQUID CROMATHOGRAPY – MASS SPECTROSCOPY WITH ELECTROSPRAY IONIZATION METHODE (LC-MS-ESI)

  1. It’s really a great and helpful piece of information. I am
    happy that you simply shared this useful info with us.
    Please stay us up to date like this. Thanks
    for sharing.

  2. bunk beds says:

    Brands last for a decade because of the quality and the service that they give to the
    user and consumer. By using these, your little boys and girls
    will be afforded more room to move about. However, what you might
    not have heard of is that over 36,000 people go to the emergency
    room every year because of bunk bed related injuries.

  3. Howdy very nice website!! Guy .. Excellent .. Wonderful ..
    I’ll bookmark your website and take the feeds also?
    I am satisfied to search out a lot of useful info here in the put up, we’d like develop more techniques on this regard, thanks for sharing.
    . . . . .

  4. Venetta says:

    I was wondering if you ever thought oof changing the structure of
    your blog? Itss very well written; I love what youve got to say.
    But maybe you could a little more in the way oof contejt so people could connet with it better.

    Youve got an awful loot of text for only having onne oor two images.
    Maybe you ciuld space it out better?

  5. Whats up very cool site!! Guy .. Excellent .. Amazing .. I’ll bookmark your blog and take the feeds also?

    I’m satisfied to find so many helpful information here in the post,
    we’d like develop more strategies in this regard, thank you for sharing.
    . . . . .

  6. วีดีโอ หนัง x ไทย
    Very descriptive article, I enjoyed that a lot. Will there be a part 2?

  7. maglia calcio, maglia calcio, nuova maglia, nuova maglia,
    maglia calcio,
    maglia calcio, maglia calcio, maglia calcio online, maglia calcio, maglia calcio,
    Jung, Jung, Jung, Jung, Jung,
    Jung, Jung, Jung, Jung, Jung,

  8. At this moment I am ready to do my breakfast, once having my breakfast coming again to read
    more news.

  9. Below are three of the several types of scopes, and they’re the most common scopes
    available on the market.

  10. Hi, I do believe this is a great site. I stumbledupon it 😉 I will come back once again since I bookmarked it.
    Money and freedom is the best way to change, may you be rich and continue to guide others.

  11. Wonderful blog! I found it while surfing around on Yahoo
    News. Do you have any suggestions on how to get listed in Yahoo News?
    I’ve been trying for a while but I never seem to get there!
    Appreciate it

  12. Excellent site you have here but I was wondering if you knew of any discussion boards that cover
    the same topics talked about here? I’d really love to be a part of group where
    I can get feedback from other experienced people that share the same
    interest. If you have any recommendations, please let me know.
    Bless you!

  13. A lot of dieters have odd habits: They attempt to trick themselves into eating unhealthy whilst believing it’s healthful.
    For instance: Some people will eat fried chicken, claiming that it’s not that high in carbohydrates and packs a lot of protein.

  14. We will discuss about fibroids in this post and if you will
    be scanning this article then you will know every one of the element of this disease.
    A high level00 woman after that this article
    is exclusively made for you. You are going to find out about the natural fibroids treatment.

    Exactly what are the main signs and symptoms of fibroids?
    The main triggers of fibroids and exactly how somebody deal better
    with fibroids?
    Fibroids as we understand only develop by the woman throughout the productive
    years. Fibroids can be described as benign tumor which occurs
    in or perhaps around the wall of uterus (Womb).
    This is definitely a kind of non-cancerous tumour which in turn usually doesn’t cause virtually
    any signs and symptoms. This can be an exceptionally common tumor all over
    the world as more than 170 million persons enduring from this.
    There are numerous female suffering from fibroids rarely know that they have got fibroids.
    You don’t need to for virtually any varieties of treatment in the event you are not
    felling any symptoms because this kind of tumour automatically shrink by simply the passing time.
    Yet for the precaution you may make the natural fibroids treatment because there are simply
    no chance of receiving the aspect effects.
    If we speak about the reasons that may lead the fibroids,
    we locate that the specific reasons for fibroids are still in the dark and no person knows the
    actual triggers of fibroids. However, we could name some reasons that may bring
    about cause the fibroids. Some sources assume that fibroids
    run in families meaning if you are battling from fibroids the probabilities are you may inherit the fibroids in your daughter.
    Several sources claim that overweight could possibly be the reason of
    fibroids.

  15. Hi, I do believe your blog could be having internet browser compatibility problems.
    Whenever I take a look at your blog in Safari,
    it looks fine however, when opening in IE, it’s got some overlapping issues.

    I merely wanted to give you a quick heads up! Aside from that, great blog!

  16. They are prised possessions of shooting enthusiasts.
    We can check out the LV bags from afar, but a top of the line Louis Vuitton goes for as much as your car,
    in all likelihood. As they are in fairly constant use, it is not really practical to fully store the bags,
    so I simply empty the contents, place in it’s own dust bag and hang
    it up.

  17. shemale cams says:

    No one ever told him to stop, as he was a
    nice guy, and he was our division officer also. •Sharing your foot fetish fantasies is another way to
    seduce a foot fetish. The greatest compliments that I have ever received have been from men who say that I make them feel
    more like a man than any other woman ever has.

  18. Mansion88 says:

    I know this if off topic but I’m looking into starting my
    own blog and was curious what all is required to get setup?
    I’m assuming having a blog like yours would cost a pretty penny?
    I’m not very internet savvy so I’m not 100% positive.
    Any tips or advice would be greatly appreciated. Appreciate it

  19. Agar.Io Hack says:

    Through such Agar io Hack, the player can get the taste of an outdoor sport in hid house and can take pleasure of the sport at any time of the day.
    Once you have a torrent client installed, you can visit any number of.
    You can also buy these older consoles for a taste of retro gaming at its finest.

  20. Nice post. I used to be checking constantly this
    blog and I’m impressed! Extremely helpful information particularly the final phase :
    ) I maintain such information much. I used to be seeking this particular info for a very lengthy time.
    Thank you and best of luck.

  21. Perhaps you can write following articles referring to this article.

  22. Winnie says:

    Thank you for the good writeup. It in fact was a amusement account
    it. Look advanced to more added agreeable from you!
    However, how can we communicate?

  23. We recommend just taking supplements that contain all-natural ingredients : ideally only HCA and
    a organic component that helps absorption, such as potassium.

  24. There is a good reason that this Sudoku game is not
    Clash Royale Hack, though. Though this app is more appropriate for kids it is nevertheless a fun way to
    learn about the states in the Union. The game is a lot
    simpler and does not have the amount of options that you come
    to expect from a realistic roulette game, but in a sense this
    approach works well for a simple pick-up-and-play style game for Android.

  25. Bonus Ebooks says:

    Good day! Would you mind if I share your blog with my facebook group?
    There’s a lot of folks that I think would really enjoy your content.
    Please let me know. Cheers

  26. Today, I went to the beachfront with my children. I
    found a sea shell and gave it to my 4 year old daughter and said “You can hear the ocean if you put this to your ear.”
    She put the shell to her ear and screamed.
    There was a hermit crab inside and it pinched her ear. She never wants to
    go back! LoL I know this is entirely off topic but I had to tell someone!

  27. Your book could be on Hippo’s but all they care about is the fact they can sell it as their own. Choose your compiler based on the necessary requirements
    for your Ebook. An e – Book reader with simple navigation controls is your best bet.

  28. Parallels Desktop for Mac from Parallels, a privately held virtualization technology company, is softgware providing hardware virtualization for Macintosh computers accessible with Intel processors.

  29. Dika says:

    salam kenal
    Mas bisakah saya bertanya tentang penentuan puncak utama dari spektrum massa dari LCMS? karena terkadang tidak hanya base peak yang dijadikan massa utama
    sebelumnya terima kasih

Leave a Reply