Liquid Chromatograpy – mass spectroscopy adalah dua  alat yang digabungkan menjadi satu, dimana berfungsi untuk memisahkan beberapa senyawa atau campuran senyawa berdasarkan kepolarannya (prinsip kerja kromatograpi), dimana setelah campuran senyawa tersebut terpisah, maka senyawa yang murni tersebut akan diidentifikasi berat moleculnya. Berbeda dengan Gas Chromatograpy – mass spectroscopy, LC-MS pada output datanya sangat jarang sekali terjadi pola fragmentasi, dikarenakan tidak ada proses fragmentasi. Sehingga yang didapatkan adalah berat molekul ditambah beberapa muatan ditambah lagi berat molekul pelarut (terkadang).

ESI adalah salah satu methode untuk mendapatkan berat molekul yang digunakan dalam LC-MS dimana jika pada metode biasanya (lupa-red) menggunakan fragmentasi (pemecahan molekul), maka pada metode ESI menggunakan spray (Penyemprotan). AKibatnya tidak akan ditemukan fragmen –fragmen dari molekul tersebut.

Adapun cara kerja liquid chromatograpi adalah sama dengan HPLC atau liquid chromatograpy lain pada umumnya. Sedangkan kerja Mass spectroscopy metode ESI  adalah sebagai berikut : 


Gambar 1. Proses pemisahan analite pada Liquid Chromatograpy sampai dengan sprayer (penyemprotan oleh spray needle tip)

1. Analyte bersama dengan eluent dari syringe pump atau LC masuk ke dalam cappilary.

Di dalam kappilary terdapat anoda (kutup negatif) pada taylor cone dan katoda (kutup negatif) didekat masukkan analyte dan eluent. Kutup ini berfungsi agar muatan yang berkumpul pada taylor cone adalah muatan positif sehingga nantinya saat terjadi penyemprotan dan terbentuk droplet (tetes – tetes) tidak bergabung – gabung menjadi droplet yang lebih besar lagi.

2.  Analylet dan solven(eluent) di semprotkan (spray) melalui taylor cone.

Akan terbentuk droplet – droplet dimana droplet – droplet itu akan mengalami tahap evaporasi solven untuk mengurangi solven yang menempel di analyte. Karena suatu saat , apabila terjadi evaporasi secara terus menerus maka solven yang meliputi analyte terkungkung dalam muatan positif yang berlebih, dalam bahasa inggris tahap seperti ini disebut the ‘rayleigh’  limit is reached, maka akan terjadi explosion yang disebut coulombic explosion dimana akan terjadi pemecahan droplet (tetesan) tadi. Ada beberapa kemungkinan yang terjadi pada droplet – droplet tersebut .

a. Yang pertama analyte akan tertambahi satu muatan positif

b. yang kedua analyte akan tertambahi beberapa muatan positif

c. yang ketiga analyte akan tertambahi satu muatan positif dan satu molekul solven

d. yang keempat analyte akan tertambahi satu muatan positif dan beberapa molekul solven

e. yang kelima analyte akant tertambahi beberapa muatan positif dan beberapa molekul solven.


Gambar 3.Proses Setelah analite dipisahkan oleh Liquid Cromatography

3. Droplet yang mengalami coulombic exsploison tersebut akan masuk ke dalam cone dimana di sisi kiri dan kanannya sudah mengalir gas Nitrogen (N2). Gas ini berfungsi agar analyte yang terjadi tadi stabil dalam bentuknya dan tidak terganggu oleh pengaruh gas oksigen. Droplet masuk ke dalam cappilary transfer lalu akan di analisis melalui mass spectrometer.


Gambar 3. Proses Droplet berubah menjadi analyte yang siap untuk di mass spectroscopy


gambar 4. Penjelasan datangnya muatan positif pada droplet – droplet LC-MS


Gambar 5. Penjelasan kenapa pada LC-MS solven harus di buat muatan positif


image gambar 6. Penjelasan kenapa bukan ion negatif yang digunakan

image gambar 7. Penjelasan ion molekul semu apa saja yang terjadi /yang bisa muncul pada output data LC-MS


gambar8. Contoh spektra dan hasil



Gambar 9. Kerugian yang dihasilkan dibandingkan dengan LC-MS


Gambar 10. Kesimpulan , diambil dari presented Jessica & Gilement, 2002.


  • Adamovics, & John.A, 1997, Chromatographic Analysis of Pharmaceutical, Marcel dekker Inc, New York.

  • Ardrey, R. E., 2003, Liquid chromatography-mass spectrometry: an introduction, 185, John Wiley & Sons, New York.

  • De Hoffmann, E., & Stroobant, V.,2007, Mass spectrometry: principles and applications, 226-227, Wiley-Interscience, New York.

  • Jesicca & Gilment, 2002, Electrospray ionization Mass Spectrometry, Presented at 17 september 2002.


  1. Great items from you, man. I have take note your stuff
    previous to and you’re just too great. I actually like what
    you have bought right here, certainly like what you are saying and the way during which you assert it.

    You make it entertaining and you continue to care for to
    stay it smart. I cant wait to read far more from you.

    That is actually a tremendous site.

  2. Isidro says:

    Paula’s Selection includes a more anti-aging items
    like the WITHSTAND Retinol Booster or the FIGHT Skin- Sculpting Minimizer repair damaged skin that I’m interested in learning too and
    to promote production.

  3. Dragon Town : An incredible Video gaming That yyou Shhould Examine

    Monster City is a sport and that is really well-known these days.
    Even so usually do not worry when it is the
    new youu find thus excellent gaming due to the fact
    we are goimg to mention that in this posting. Though
    the main purpose with this content would be to show you how to get no cost important jewels foor this specific
    great sport. And you will probably easily recognize exactly how cool this video game is usually.

    A spot for you to interact with — that has been the exact belief for
    this inventor of FACEBOOK OR TWITTER after this individual
    started off generating this website. On the other hand there seemed to be no potential for an affiliate site similar to
    this to obtain favorite. Consequently, your originator affiliated utilizing this type of web sire
    put on video game titles that granted people in order to
    speak with one another in addition to engzge in online games simultaneously.

    There are several fitms in which created their
    unique online games easily obtainable in this kind off
    community and so are today well-liked. Plus Dragon Metropolis is usually a game whyich in turn obbtained plenty of name simply speaking time frame.
    It is time for you to consider exactly what this specific game is providing.

    Monster Location is usually involving by far the most entertaining game
    titles on the net. However it really is additionally involved combined with Facebook or myspace.

    Therefore you could engage in this with your buddies though aiding
    the other. That’s a fascinating activity in which anyone nurture ones dragons just
    a excellent world filled up with island destinations.

    You possibly can moreover combat against several other avid gamers in the
    area and whom functions the most effective mythc beasts
    team. Come up with a great earth loaded with actions.
    Receive lots of dragons, replicate and degree upward
    these phones end up being valued as a possible specialist involving dragons.
    After you will be fixed, you are going too bbe able to struggle in direction of all avid gamers.

    Nonetheless this kind of online game has become additionally readily available for cellphones, probably not only in Facebook or
    myspace per se. You’ll can be usd too participate in the particuoar sport at any place you want.

    Lots of merhods will be important in the event you would like to swiftly
    educate your mythic monsters. That’s why we have been in thijs article to make your own personal Monster Citty time consideraboy more easy by way of a created specifically dragon City hack application that can make available to you
    unrestricted usage of normal methods along with most which usually you will need on this unique sport.
    To be a Monster Grasp here is easy and also risk-free and
    it’s a new merely number of clicks aside only if you’ll be making use of dragon city
    cheats which have been supplied coming from dragon City hack instrument.
    Combating market is certainly anticipating you — keep in mind to utilize dragon Ciity hack
    In addition, I must indicate that a page might by no means find banned by using this specific dragon City hack device.
    Regular updates are provided to the present specific dragon City hack software.

    Which assures of which dragon city cheats will likely be working.
    Just what are yyou searching forward to help? Make use of dragon city cheats along with take advantazge of the online game towards fullest level.

  4. Eugenia says:

    Unquestionably believe that which you said.
    Your favorite justification seemed to be on the web the simplest thing
    to be aware of. I say to you, I definitely get annoyed while people think
    about worries that they plainly do not know about.

    You managed to hit the nail upon the top and also defined out the whole thing without having side effect , people could take a signal.
    Will probably be back to get more. Thanks

  5. Dragon Town — A fantastic Video gaming Yoou will Must Take a look at

    Dragon City can be a activity that is genuinely well-known right now.

    Even so won’t stress in the event that oahu is the first-time you
    see this unique video gaming simply because we
    willl refer to the item on this page. Thoughh the main gal of this article is usually too explain how to gett free of charge valuable gemstones for this certain great activity.
    And you will rapidly acknowledge the best wway awesome thjs specific online game ccan be.

    An area to be able to connect to – which was the exact perception associated with the originator of ZYNGA the moment this individual started generating this site.
    On tthe other hand there was clearly noo risk of an affiliate site similar to this to have
    well-known. Thus, this originator connected on this website put on game titles which
    in turn granted persons for you to talk with one
    another along with lay flash games in unison. There are numerous
    organizattions of which made their unique online flash games accessible in this ype of local community annd so are today popular.
    And in addition Dragon City cann be a sport which usually obtained
    plenty of standing in other words period of time. It can be time
    and energy to check into just the thing this
    kind of gaming iis offering.
    Monster City is among essentially the most exciting games on-line.
    Although it happens to be also involved as well as Myspace.
    This means that you could play the item with all your
    pals although helping one another. That is the amazing
    sport during which anyone nurtufe your own dragons inside
    a fantastic globe full of countries. You possibly can in addition fight versus many other
    gaame enthusiasts inside a area and see who capabilities the most
    efficient mythic critters staff. Come up with a wonderful globe packed with routines.
    Acquire a great deal of dragons, replicate along with amount
    upward them to be sought after as an professional associated with
    dragons. After you happen to be collection, you’ll
    possibly be in a position to battle toward all participants.
    Even soo that activity actually iis furthermore intended for
    mobile phone devices, certainly not only in Zynga themselves.
    You will cann easily engage in the particular adventure at any plae you’llwant.

    A great deal oof sources shall be essential if perhaps you waqnt to speedily train your mythic monsters.
    That’s why we’re in this article to produce
    your individual Monster City evening much more simple using a created dragon City hack
    tool that may make available to you unlimited having access to natural means in addition to
    juhst about all which often you will need within this distinct sport.
    As a Monster Learn at this point iis not hard and as well risk-free and a nnew simply handful of clicks of the
    mouse to one side only if you’ll be employing
    dragn cigy cheats which have been supplied vvia dragon City hck application. Fighting wofld is unquestionably looking forward to an individual –
    remember to make use of dragon City hack apk.

    Additionally, I have to talk about that your particular
    report might never ever receive forbidden by utilizing this kind of dragon City hack
    tool. Continuous improvements are supplied to the certain dreagon City hack application.
    In which ensures of which dragon city cheats are going to be running.
    Just what exactly looking forwards to be able to? Make use of draqgon city
    cheat and benefit from the sport for the fullest extent degree.

  6. My coder is trying to persuade me to move to .net from PHP.

    I have always disliked the idea because of the costs.
    But he’s tryiong none the less. I’ve been using WordPress on a number of websites for about a year and am nervous about
    switching to another platform. I have heard good things about

    Is there a way I can import all my wordpress content into it?
    Any kind of help would be greatly appreciated!

  7. Because I get up with the damn anxiety each day and begin pushing by it as I can.

  8. Despite this, we still find it fair to say that there is inconsistent portrayal of behavioral research across the institutes and centers of NIH.

  9. This is likely to present long term research areas of
    interest to several stakeholders.

  10. Kendra says:

    Once Camille was out of the woods and secure after her
    second surgical procedure, she had loads of time to
    think and he or she realized that she was in love with someone else, so she left Mike.

    It is sad, however it occurs. It was clear right off the bat
    that The Art of Blowjob would have to change because
    it could now not really feel right for them to make porn collectively.
    As for , that was more unclear.

    Ultimately, she determined to do her personal thing along with
    her new associate. We respect her resolution and want her one of the best.

  11. Its like you read my mind! You appear to know so much about this,
    like you wrote the book in it or something. I think that you can do
    with a few pics to drive the message home a bit, but other than that, this is great blog.
    A fantastic read. I will definitely be back.

  12. summer says:

    Wow, amazing weblog format! How lengthy have you been running a blog for?

    you make blogging glance easy. The overall
    look of your website is excellent, let alone the content material!

  13. I have been surfing online greater than three
    hours as of late, yet I by no means found any attention-grabbing article like yours.
    It’s pretty price enough for me. In my opinion, if all webmasters
    and bloggers made good content material as you did, the internet shall be a
    lot more helpful than ever before.

  14. If you are going for best contents like myself, simply pay a quick
    visit this site everyday becsuse it offers feature contents, thanks

  15. I am basically satisfied with this particular website and that I
    wanted to take the time to many thanks in the bottom of my heart!

  16. Great topic and well written. Do you have any more resources about this that you

  17. Great topic and well written. Do you have any more resources about this that
    you reccommend?

  18. Great topic and well written. Do you have any more resources
    about this that you reccommend?

  19. We not only have the best quality mlb, nhl, ncaa, nba jerseys, but also cheap wholesale nba basketball jerseys,
    come and have a look.

  20. The imagery may be different on your deck,
    but the meanings will be the exact same. This can manifest as theatre and movie work, creating fiction, poetry or portray.
    Will I contact Leonora, our animal communicator, again?

  21. Great topic and well written. Do you have any more resources about this that you reccommend?

  22. หนัง says:

    Hi there, I found your web site by the use of Google while looking for a similar topic,
    your site got here up, it appears great. I’ve bookmarked it in my google bookmarks.

    Hello there, just become alert to your weblog thru Google, and located that it
    is truly informative. I’m gonna be careful for brussels.

    I’ll appreciate in case you continue this in future.
    Numerous other folks will be benefited from your writing.

  23. Its like you read my mind! You appear to know so much about this, like you wrote the book in it or something.

    I think that you can do with some pics to drive the message
    home a bit, but other than that, this is excellent
    blog. An excellent read. I’ll certainly
    be back.

  24. FU says:

    HPU’s Chief Financial Officer approaches yoou with the question, “In which judicial forum should HPU file a lawsuit against the IRS: (1) U. Painting: Whether for interiors or exteriors knowing the best way to paint is a great skill to have as hiring others to do the work can sometimes cost thousands of dollars. Sometimes taking unnecessary short cuts to save money will not prove to be helpful to get best home improvement results.

  25. Just after the mold removal you ought to throw away the clothes or
    else wash them straight away.

  26. Charli says:

    Hello! This is my first visit to your blog! We are a team
    of volunteers and starting a new initiative in a community in the same niche.
    Your blog provided us useful information to work on. You have done a outstanding

  27. No one ever told him to stop, as he was a nice guy, and he was our division officer also.
    It gives you a chance to relax, slow down and admire every each of each other’s bodies.
    The shemale escorts in London are not males but females.

  28. says:

    Great topic and well written. Do you have any more resources about this that you reccommend?

  29. pobierz says:

    Saved as a favorite, I like your blog!

  30. Select cheap jordans models you like! Air Jordan Shoes Outlet owns full sizes for men,women and kids,
    mostly are for sale now!
    More from:Cheap Jordans – Air Jordan Shoes Outlet Sale

  31. always i used to read smaller articles that as well clear their motive, and
    that is also happening with this paragraph which I am reading now.

  32. Theda says:

    Alicia claims that the skin does not have the identical microorganisms as the stomach, therefore the ingredients have to be stabilized, helping to make the process

  33. pobierz says:

    Your way of explaining all in this post is in fact
    pleasant, every one be able to effortlessly be aware
    of it, Thanks a lot.

  34. Heya i am for the first time here. I found this board and I find It truly useful & it helped me out a lot.
    I hope to give something back and help others like you helped me.

  35. beding says:

    Its such as you learn my mind! You seem to understand a lot approximately this, such as you wrote the book in it
    or something. I feel that you just can do with some p.c.
    to drive the message home a little bit, but other than that, this
    is great blog. A fantastic read. I will certainly be back.

  36. These are truly impressive ideas in regarding blogging.
    You have touched some pleasant points here. Any way keep up wrinting.

  37. In any platform (win, mac pc) orr device(iOS, Google android)
    you are palying mobile strike, right now you can add the assets!

  38. Tommie says:

    I do not even understand how I ended up right here, but I thought this post was good.

    I don’t know who you are however certainly you’re going to a famous blogger when you aren’t already.

  39. tribratanews says:

    I like it when individuals get together and share opinions.
    Great blog, keep it up!

  40. I loved your blog.Really thank you! Awesome.

  41. Audrey says:

    Spot on with this write-up, I honestly believe that this
    web site needs a great deal more attention. I’ll probably be returning
    to read through more, thanks for the info!

  42. Oh my goodness! an incredible article man. Thank you for sharing

  43. You will discover millions of men and women around the world who don’t find out anything
    about Psoriasis since this disease as certainly not
    common as other disorders such as HIV supports, Hepatitis B, Cancer, WARTS
    and diabetes. However, this kind of disease continually spreading all their feet on alarming price around the globe.
    So, it can be very important to include each of
    the information regarding the psoriasis. Since you are in this article reading this then you
    are at the best location to gather all the essential information regarding
    the Psoriasis. By gathering this article’s information it will be possible to consider appropriate steps to quit spreading this
    Psoriasis is a non-contagious virus-like skin infection which
    can easily affect any parts of the body. Psoriasis arises when our immune program gives
    faulty signals to our brain which bring about the
    high growth charge of the dead pores and skin cells on the afflicted area.

    This is a common disease around the globe specifically in western countries.

    We should speak about the causes that may bring about development of
    the psoriasis. Scientists have not yet recognized the specific causes of the
    psoriasis but some probable triggers which may bring about this kind of infection. Generally researchers
    believe this infection most typically runs in families. This kind of is the most possible factor that
    can become bring about the psoriasis. A lot of scientists
    assume that a person suffering from HIV could possibly get psoriasis while others feel that heavy alcohol consumption can be
    the key reasons of the development of the psoriasis. Many assume that acquiring heavy medicines could end up being the reason from the expansion of the Psoriasis.

    Smoking cigarettes could be the different reason from the development of the psoriasis.
    Let get honest, all the in this article mentioned reasons are only
    based on assumptions even so; these reasons could get somehow relate to the illness.

  44. Thank you ever so for you article. Awesome.

  45. Great, thanks for sharing this article post.Thanks Again. Keep writing.

  46. Contact: 866-385-7441

    If you have an irs bank levy or an irs wage levy. Release that irs
    levy together with the assist of a tax attorney who can get
    a release of tax lien by way of an offer in compromise with the IRS.

  47. rings online says:

    When Kris popped question to Kardashian in front of her Beverly Hiills home on May 18, she described the experience as ,
    ‘I didn’t expect tthis at all. The $500,000 diazmond engagement ring tied a knot between Brad Pitt and Jennifer Aniston. Due to their eleganbt hues annd
    dazzling aesthetic, garnet engagement rings aare items that will aways be appreeciated and adored by tthe wearer.

  48. Hello would you mind sharing which blog platform
    you’re working with? I’m planning to start my
    own blog in the near future buut I’m having a difficult time selecting between BlogEngine/Wordpress/B2evolution and Drupal.
    The reason I ask is because your design seems different then most blogs and I’m looking for something unique.

    P.S My apologiees for getting off-topic but I
    had to ask!

  49. Appreciate the recommendation. Will try it out.

Leave a Reply