oke, kali ini andy akan menjawab pertanyaan seorang kaskuser yang bertanya tentang perbedaan Liquid Cromatography Mass Spectroscopy menggunakan metode APCI (atmospheric-pressure chemical ionization) dan metode ESI (electrospray ionization). Jika temen – temen masih kurang tahu tentang apa itu ESI, bisa di baca disini (materi 1) dan disini (materi 2).

image

 

Intermezo bagi yang masih amatir tentang liquid chromatograpy

ehm,…aku neranginnya pake bahasa inggris ya, nyoba sedikit – sedikit.

first,

Liquid chromatograpy-mass spektroscopy is analytical instrument used identification structure chemestry a compound (benerke nek salah!!).

in LC-MS ada beberapa metode untuk mengionisasi compound tersebut sehingga nantinya siap terdeteksi di dalam mass spekstroskopy berat molekul dari ion – ionnya baik sudah terfragmentasi, maupun molekul utuh yang bisa saja tertambahi oleh pelarut atau ion semu.(heheehe).

ionization methodes that may be utilized in LC-mS include electron ionization
(EI), chemical ionization (CI), fast-atom bombardment (FAB), thermospray
(TSP), electrospray (ESI) and atmospheric-pressure chemical ionization (APCI).

terjemahannya secara kasar gini:

metode ionization yang dimanfaatkan dalam LC-MS termasuk EI, CI, FAB, TSP, ESI, dan APCI

nah…kali ini, bahasan yang akan kita bahas adalah perbedaan ESI dan APCI

 

Pada Atmospheric-pressure chemical ionization (APCI) aliran cairan (didalamnya adalah solven dan senyawa identifikasi)  yang berasal dari kolom HPLC di dispersikan kedalam droplet yang sangat kecil, dalam hal ini dikombinasikan dengan panas dan gas nebulizing, sedangkan ESI tidak.

image

 

Perbedaan secara teknisnya ESI digunakan untuk analyte yang memiliki polaritas medium sampai high sedangkan APCI untuk anality low sampai medium.

ESI memiliki perbedaan yang signifikan daripada APCI dalam hal sensitiftas dan juga lebih disarankan untuk penggunaan secara kuantitatif

 

sekian gan! informasi dariku…jika ada pertanyaan , mungkin aku bisa bantu lagi.

DAFTAR PUSTAKA

 

Ardrey, Liquid Chromatography Mass spectroscopy an introduction, Wiley interscience.

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